Reconstitution calculator · SS-31

SS-31 reconstitution calculator.

Work out the concentration of a reconstituted SS-31 vial. Presets match the 10 mg / 50 mg formats we stock, and the reference table below is calculated for those exact vials. Research use only.

Vial size

This tool reports concentration only. It is not dosing guidance, and nothing here is for human use.

SS-31 concentration reference table

Every combination of the SS-31 vial sizes we stock and the bacteriostatic water volumes people most often add.

VialBAC waterConcentrationPer 100 µL aliquot
10 mg1 ml10 mg/ml1 mg (1,000 mcg)
10 mg2 ml5 mg/ml0.5 mg (500 mcg)
10 mg3 ml3.333 mg/ml0.333 mg (333 mcg)
10 mg5 ml2 mg/ml0.2 mg (200 mcg)
50 mg1 ml50 mg/ml5 mg (5,000 mcg)
50 mg2 ml25 mg/ml2.5 mg (2,500 mcg)
50 mg3 ml16.667 mg/ml1.667 mg (1,667 mcg)
50 mg5 ml10 mg/ml1 mg (1,000 mcg)

How the calculation works

Concentration is the mass of peptide divided by the volume of solvent. A vial holding 5 mg reconstituted with 2 ml of bacteriostatic water gives 2.5 mg/ml, so a 100 µL aliquot contains 0.25 mg. The powder itself adds no meaningful volume at these masses, which is why the water volume alone sets the concentration.

Handling in the Vietnamese climate

Sealed lyophilized vials are stable at room temperature in transit, which is why the material ships dry rather than cold. Once reconstituted, the solution is kept refrigerated and shielded from light. Bacteriostatic water is the usual solvent because the benzyl alcohol in it lets a vial be entered more than once.

What is SS-31?

A tetrapeptide that targets a specific lipid inside the mitochondria.

SS-31, also known as elamipretide, is a four-amino-acid peptide with an unusual property: it reaches a specific location. Most molecules diffuse where chemistry takes them, but SS-31 carries an alternating charge pattern that concentrates it in the inner mitochondrial membrane.

There it binds cardiolipin, a lipid unique to that membrane and essential for organising the machinery that generates cellular energy. When cardiolipin becomes disorganised, energy production falters. Which is why SS-31 is one of the more clinically advanced compounds in this catalog, having reached human trials for mitochondrial conditions.

Research context

Type
Tetrapeptide (4 amino acids)
Also known as
Elamipretide
Mechanism of interest
Concentrates in the inner mitochondrial membrane and binds cardiolipin
Format
Lyophilized powder in a sealed vial
Handling
Sealed vials are kept cold and out of direct light. Relevant in the Vietnamese climate
SS-31 product page

Common questions

What is SS-31?

A four-amino-acid peptide, also known as elamipretide, whose alternating charge pattern concentrates it in the inner mitochondrial membrane, where it binds the lipid cardiolipin. Listed here as a non-clinical research material.

Why does cardiolipin matter?

Cardiolipin is a lipid unique to the inner mitochondrial membrane, and it organises the machinery that produces cellular energy. When it becomes disorganised, energy production falters. Which is the basis of research interest in SS-31.

How much bacteriostatic water do I add to a SS-31 vial?

There is no single correct volume. The water volume sets the concentration: more water gives a weaker solution and a larger aliquot for the same mass. Common choices are 1 to 5 ml. Enter your vial size above to see what each volume produces for 10 mg / 50 mg.

What concentration does a SS-31 vial give?

It depends entirely on the solvent volume. The reference table above lists every combination of the 10 mg / 50 mg vial format and 1, 2, 3 and 5 ml of bacteriostatic water.

Does the powder change the final volume?

Not measurably at these masses. A few milligrams of lyophilized peptide occupies a negligible volume, so the bacteriostatic water you add is effectively the final volume.

Can I use normal water or saline instead?

Bacteriostatic water is the standard solvent for multi-entry vials because it contains a preservative. Sterile water and saline have no preservative, so they are single-entry solvents.

Check SS-31 availability

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