Free tool · No sign-up · Research use only
Peptide reconstitution calculator.
Enter the peptide mass in the vial and the volume of bacteriostatic water added. The calculator returns mg/ml, mcg/ml and the amount contained in each standard laboratory aliquot. It does not choose a solvent, protocol or human-use amount.
The formula
- Concentration
- vial mg ÷ water ml = mg/ml
- Example
- 5 mg ÷ 2.5 ml = 2 mg/ml
- 100 µL aliquot
- 0.2 mg (200 mcg)
- Scope
- Research-use laboratory arithmetic
Calculator.
Pick a common vial size or type your own laboratory values.
Research-use only. This tool performs arithmetic; it does not provide a protocol, dosing, injection or self-administration instructions.
What the tool calculates.
Reconstitution concentration is the peptide mass in the vial divided by the volume of bacteriostatic water added. A 5 mg vial reconstituted with 2.5 ml gives 2 mg/ml; the same vial with 5 ml gives 1 mg/ml. More water means a lower concentration and a larger measured volume per aliquot.
The aliquot rows are simple volume conversions for experiment planning. They are not recommendations. Use only a validated laboratory protocol for solvent, handling and storage conditions. Bacteriostatic water is listed in the catalog alongside the peptides.
How to reconstitute a peptide vial, step by step.
A clear guide for research planning. Use the calculator above to determine your concentration, then follow these steps.
You need: the peptide vial, a vial of bacteriostatic water, an alcohol swab, and an appropriate mixing syringe. Everything should be at the protocol-specified temperature before starting.
Use the calculator above. Enter the peptide mass in the vial and the volume of bacteriostatic water you plan to add. The calculator gives you the mg/ml concentration and the amount in each standard aliquot. So you know exactly what each measured volume contains.
Clean the vial stopper with an alcohol swab. Draw the calculated volume of bacteriostatic water into the syringe. Pierce the stopper at an angle and slowly drip the water onto the inner wall of the vial. This prevents foaming and preserves the peptide structure.
Roll the vial slowly between your fingers. Let it rest for a few minutes until all powder has dissolved and the liquid is completely clear. Never shake the vial, shaking can denature the peptide.
Store the reconstituted peptide at the temperature specified in your research protocol. Most peptides require refrigeration (2–8°C) and protection from light. Check the specific storage requirements for your research material.
Need supplies? Bacteriostatic water, syringes and alcohol swabs are available in VND or USD. Check the catalog or message @vietpeptides.
FAQ: reconstitution math.
How do you calculate peptide reconstitution concentration?
Divide the peptide mass in the vial by the volume of bacteriostatic water added. For example, a 5 mg vial with 2.5 ml gives 2 mg/ml, so a 100 microliter laboratory aliquot contains 0.2 mg.
How much bacteriostatic water should be added?
That volume comes from the laboratory protocol, not from this tool. More water gives a lower mg/ml and a larger measured volume per aliquot; less water gives a higher mg/ml. Enter the protocol values and the calculator returns the resulting concentration.
What volumes does the calculator show?
It shows the material contained in 50, 100, 250 and 500 microliter laboratory aliquots. These are arithmetic conversions for research planning.
Does it choose a solvent or protocol?
No. Use the solvent, concentration and handling procedure specified by an authorized laboratory protocol.
Is this calculator for human use?
No. It is a laboratory concentration calculator for research use only. It provides no dosing, injection or self-administration guidance.