Reconstitution calculator · TB-500
TB-500 reconstitution calculator.
Work out the concentration of a reconstituted TB-500 vial. Presets match the 10 mg format we stock, and the reference table below is calculated for those exact vials. Research use only.
This tool reports concentration only. It is not dosing guidance, and nothing here is for human use.
TB-500 concentration reference table
Every combination of the TB-500 vial sizes we stock and the bacteriostatic water volumes people most often add.
| Vial | BAC water | Concentration | Per 100 µL aliquot |
|---|---|---|---|
| 10 mg | 1 ml | 10 mg/ml | 1 mg (1,000 mcg) |
| 10 mg | 2 ml | 5 mg/ml | 0.5 mg (500 mcg) |
| 10 mg | 3 ml | 3.333 mg/ml | 0.333 mg (333 mcg) |
| 10 mg | 5 ml | 2 mg/ml | 0.2 mg (200 mcg) |
How the calculation works
Concentration is the mass of peptide divided by the volume of solvent. A vial holding 5 mg reconstituted with 2 ml of bacteriostatic water gives 2.5 mg/ml, so a 100 µL aliquot contains 0.25 mg. The powder itself adds no meaningful volume at these masses, which is why the water volume alone sets the concentration.
Handling in the Vietnamese climate
Sealed lyophilized vials are stable at room temperature in transit, which is why the material ships dry rather than cold. Once reconstituted, the solution is kept refrigerated and shielded from light. Bacteriostatic water is the usual solvent because the benzyl alcohol in it lets a vial be entered more than once.
What is TB-500?
A synthetic 7-amino-acid fragment of the protein thymosin beta-4.
TB-500 is not thymosin beta-4 itself, and the distinction matters. Thymosin beta-4 is a 43-amino-acid protein present in most cells and in wound fluid. TB-500 reproduces only the short active region of it. The seven amino acids that bind actin, one of the main structural proteins inside cells.
Actin binding is the mechanism behind most research interest: it relates to how cells move and reorganise. The published record is animal work, largely in connective tissue and cardiac models.
Research context
- Actin binding and cell motility
- Connective tissue models in animals
- Angiogenesis research
- Type
- Peptide, 7 amino acids (thymosin beta-4 fragment)
- Origin
- Synthetic fragment of the natural 43-amino-acid protein
- Mechanism of interest
- Binds actin, a structural protein inside cells
- Format
- Lyophilized powder in a sealed vial
- Handling
- Sealed vials are kept cold and out of direct light. Relevant in the Vietnamese climate
Common questions
What is TB-500?
A synthetic 7-amino-acid peptide reproducing the active actin-binding region of thymosin beta-4, a 43-amino-acid protein found in most cells. Listed here as a non-clinical research material.
Is TB-500 the same as thymosin beta-4?
No. Thymosin beta-4 is the full 43-amino-acid protein; TB-500 is only its short active fragment. They are often used interchangeably in casual discussion, which is not accurate.
How much bacteriostatic water do I add to a TB-500 vial?
There is no single correct volume. The water volume sets the concentration: more water gives a weaker solution and a larger aliquot for the same mass. Common choices are 1 to 5 ml. Enter your vial size above to see what each volume produces for 10 mg.
What concentration does a TB-500 vial give?
It depends entirely on the solvent volume. The reference table above lists every combination of the 10 mg vial format and 1, 2, 3 and 5 ml of bacteriostatic water.
Does the powder change the final volume?
Not measurably at these masses. A few milligrams of lyophilized peptide occupies a negligible volume, so the bacteriostatic water you add is effectively the final volume.
Can I use normal water or saline instead?
Bacteriostatic water is the standard solvent for multi-entry vials because it contains a preservative. Sterile water and saline have no preservative, so they are single-entry solvents.
Check TB-500 availability
Send the product and format in Telegram. Availability and the total are confirmed in writing before payment.