Reconstitution calculator · Glutathione

Glutathione reconstitution calculator.

Work out the concentration of a reconstituted Glutathione vial. Presets match the 1500 mg format we stock, and the reference table below is calculated for those exact vials. Research use only.

Vial size

This tool reports concentration only. It is not dosing guidance, and nothing here is for human use.

Glutathione concentration reference table

Every combination of the Glutathione vial sizes we stock and the bacteriostatic water volumes people most often add.

VialBAC waterConcentrationPer 100 µL aliquot
1500 mg1 ml1,500 mg/ml150 mg (150,000 mcg)
1500 mg2 ml750 mg/ml75 mg (75,000 mcg)
1500 mg3 ml500 mg/ml50 mg (50,000 mcg)
1500 mg5 ml300 mg/ml30 mg (30,000 mcg)

How the calculation works

Concentration is the mass of peptide divided by the volume of solvent. A vial holding 5 mg reconstituted with 2 ml of bacteriostatic water gives 2.5 mg/ml, so a 100 µL aliquot contains 0.25 mg. The powder itself adds no meaningful volume at these masses, which is why the water volume alone sets the concentration.

Handling in the Vietnamese climate

Sealed lyophilized vials are stable at room temperature in transit, which is why the material ships dry rather than cold. Once reconstituted, the solution is kept refrigerated and shielded from light. Bacteriostatic water is the usual solvent because the benzyl alcohol in it lets a vial be entered more than once.

What is Glutathione?

The cell's main antioxidant. A tripeptide of glutamate, cysteine and glycine, present in every living cell.

Glutathione is not a designed research peptide at all: it is a tripeptide the body makes constantly, built from glutamate, cysteine and glycine, and held inside cells at millimolar concentrations. It was first described in 1888 and its structure worked out by Frederick Hopkins, which places it among the oldest molecules in this catalog by a wide margin.

Its chemistry rests on one cysteine thiol group. That -SH group is what neutralises reactive oxygen species, what glutathione peroxidase uses as a cofactor, and what glutathione-S-transferases attach to foreign compounds during phase II metabolism. The ratio between reduced glutathione and its oxidised dimer (GSH/GSSG) is a standard laboratory readout for oxidative stress. Because oral glutathione is largely broken down before absorption, research work uses vial formats instead.

Research context

Type
Tripeptide, glutamate, cysteine, glycine
Not
Not a hormone and not a designed analog; endogenous to every cell
Active chemistry
The cysteine thiol (-SH) group
History
First described 1888; structure established by Frederick Hopkins
Format
Lyophilized powder vial, 1500 mg
Glutathione product page

Common questions

What is Glutathione?

A tripeptide made of glutamate, cysteine and glycine that every living cell produces and uses as its principal antioxidant. This vial is a research-grade material, listed for laboratory work only, not for human use.

Why is glutathione supplied in a vial rather than as a capsule?

Oral glutathione is largely digested before it reaches circulation, so the intact tripeptide is hard to deliver that way. Laboratory work uses a reconstituted vial format for that reason. This is a note on why the format exists, not any guidance on use.

How much bacteriostatic water do I add to a Glutathione vial?

There is no single correct volume. The water volume sets the concentration: more water gives a weaker solution and a larger aliquot for the same mass. Common choices are 1 to 5 ml. Enter your vial size above to see what each volume produces for 1500 mg.

What concentration does a Glutathione vial give?

It depends entirely on the solvent volume. The reference table above lists every combination of the 1500 mg vial format and 1, 2, 3 and 5 ml of bacteriostatic water.

Does the powder change the final volume?

Not measurably at these masses. A few milligrams of lyophilized peptide occupies a negligible volume, so the bacteriostatic water you add is effectively the final volume.

Can I use normal water or saline instead?

Bacteriostatic water is the standard solvent for multi-entry vials because it contains a preservative. Sterile water and saline have no preservative, so they are single-entry solvents.

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