Reconstitution calculator · Thymosin Alpha-1
Thymosin Alpha-1 reconstitution calculator.
Work out the concentration of a reconstituted Thymosin Alpha-1 vial. Presets match the 5 mg format we stock, and the reference table below is calculated for those exact vials. Research use only.
This tool reports concentration only. It is not dosing guidance, and nothing here is for human use.
Thymosin Alpha-1 concentration reference table
Every combination of the Thymosin Alpha-1 vial sizes we stock and the bacteriostatic water volumes people most often add.
| Vial | BAC water | Concentration | Per 100 µL aliquot |
|---|---|---|---|
| 5 mg | 1 ml | 5 mg/ml | 0.5 mg (500 mcg) |
| 5 mg | 2 ml | 2.5 mg/ml | 0.25 mg (250 mcg) |
| 5 mg | 3 ml | 1.667 mg/ml | 0.167 mg (167 mcg) |
| 5 mg | 5 ml | 1 mg/ml | 0.1 mg (100 mcg) |
How the calculation works
Concentration is the mass of peptide divided by the volume of solvent. A vial holding 5 mg reconstituted with 2 ml of bacteriostatic water gives 2.5 mg/ml, so a 100 µL aliquot contains 0.25 mg. The powder itself adds no meaningful volume at these masses, which is why the water volume alone sets the concentration.
Handling in the Vietnamese climate
Sealed lyophilized vials are stable at room temperature in transit, which is why the material ships dry rather than cold. Once reconstituted, the solution is kept refrigerated and shielded from light. Bacteriostatic water is the usual solvent because the benzyl alcohol in it lets a vial be entered more than once.
What is Thymosin Alpha-1?
A 28-amino-acid peptide isolated from the thymus gland, studied for its role in immune signalling.
Thymosin alpha-1 is a naturally occurring 28-amino-acid peptide, acetylated at its N-terminus and cleaved from a larger precursor protein called prothymosin alpha. Allan Goldstein's group first isolated it from calf thymus extract in the 1970s while working out which fraction of thymus tissue carried its immune activity.
Research interest centres on how it influences immune cell behaviour rather than on suppressing or stimulating the system bluntly. Published work looks at dendritic cell maturation, T-cell differentiation and TLR9 signalling. Under the name thymalfasin it is a licensed medicine in a number of countries, though not in the United States. Do not read that as an endorsement of this listing: this vial is a research material, and nothing on this page is medical guidance.
Research context
- Dendritic cell maturation and T-cell differentiation
- TLR9 and innate immune signalling pathways
- Immune modulation in in-vitro and animal models
- Type
- Naturally occurring peptide, 28 amino acids, N-terminally acetylated
- Origin
- First isolated from calf thymus extract, Goldstein's group, 1970s
- Precursor
- Cleaved from prothymosin alpha
- Also known as
- Thymalfasin. A licensed medicine in some countries, not in the US
- Formats
- Lyophilized powder vials, 5 mg and 10 mg
Common questions
What is Thymosin Alpha-1?
A 28-amino-acid peptide that occurs naturally in the thymus gland, first isolated in the 1970s and studied for its effect on immune cell signalling. Listed here as a non-clinical research material only, not for human use.
Is Thymosin Alpha-1 the same as TB-500?
No, and the shared word is misleading. TB-500 is a fragment of thymosin beta-4, an actin-binding protein studied in tissue research. Thymosin alpha-1 is a separate 28-amino-acid peptide from a different precursor, studied in immune research. They are unrelated molecules that happen to share a family name.
How much bacteriostatic water do I add to a Thymosin Alpha-1 vial?
There is no single correct volume. The water volume sets the concentration: more water gives a weaker solution and a larger aliquot for the same mass. Common choices are 1 to 5 ml. Enter your vial size above to see what each volume produces for 5 mg.
What concentration does a Thymosin Alpha-1 vial give?
It depends entirely on the solvent volume. The reference table above lists every combination of the 5 mg vial format and 1, 2, 3 and 5 ml of bacteriostatic water.
Does the powder change the final volume?
Not measurably at these masses. A few milligrams of lyophilized peptide occupies a negligible volume, so the bacteriostatic water you add is effectively the final volume.
Can I use normal water or saline instead?
Bacteriostatic water is the standard solvent for multi-entry vials because it contains a preservative. Sterile water and saline have no preservative, so they are single-entry solvents.
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